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2026 Vol.36, Issue 9 Preview Page

Research Paper

Application of Ribose-EDC/NHS Dual Crosslinking to Improve Functional Stability of Porcine Skin-Derived Collagen Barrier Membranes

돼지 피부 유래 콜라겐 차폐막의 기능적 안정성 향상을 위한 리보스-EDC/NHS 이중 가교의 적용

Hyun Su Park12
,
Tae Ryeol Kim2
,
Su Jeong Lim1
,
Ji Eun Kim1
,
Hee Jin Song1
,
Ayun Seol1
,
Ye Eun Ryu1
,
Ye Ryeong Kim1
,
You Jeong Roh2
,
Ji Yun Yun2
,
Byoung Soo Kim2
,
Dae Youn Hwang1†
박 현수12
,
김 태렬2
,
임 수정1
,
김 지은1
,
송 희진1
,
설 아윤1
,
류 예은1
,
김 예령1
,
노 유정2
,
윤 지윤2
,
김 병수2
,
황 대연1†
1Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea
2DENTIS CO., LTD., Daegu 41065, Republic of Korea
1부산대학교 생명자원과학대학 바이오소재과학과
2(주)덴티스
†Corresponding author E-Mail : dyhwang@pusan.ac.kr (D. Y. Hwang, Pusan Nat’l Univ.)
27 September 2026. pp. 325-339
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Abstract

Collagen barrier membranes are widely used in guided tissue regeneration (GTR) and guided bone regeneration (GBR), but extracted collagen-based membranes can show limited mechanical stability and rapid enzymatic degradation. In this study, we investigated whether ribose-1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) dual crosslinking improves the functional stability of a porcine skin-derived collagen barrier membrane. To achieve this, optimal conditions for dual crosslinking were established, and these conditions were applied to produce a ribose-EDC/NHS dual-crosslinked collagen barrier membrane (RENC) using a non-crosslinked collagen membrane (COL). Then their mechanical and physicochemical properties and biocompatibility were analyzed. RENC showed a denser surface morphology than COL while retaining major collagen-related amide band patterns. RENC also exhibited distinctive thermal behavior compared to COL and improved tensile properties. After collagenase and trypsin treatment, RENC showed lower weight loss than COL, indicating enhanced resistance to enzymatic degradation. In L-929 cell-based assays, RENC maintained cytocompatibility without inducing apparent morphological changes. In the subcutaneous implantation rat model, RENC gradually degraded over time. Residual membrane area tended to decrease over time in both RENC and a bovine pericardium-derived barrier membrane (BP), and no necrosis or other adverse local tissue reactions were observed in either group. These results suggest that ribose-EDC/NHS dual crosslinking may be a useful strategy for improving the functional stability of extracted collagen-based barrier membranes.

Keywords
collagen barrier membrane
ribose-EDC/NHS
dual crosslinking
enzymatic degradation
functional stability
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Information
  • Publisher :Materials Research Society of Korea
  • Publisher(Ko) :한국재료학회
  • Journal Title :Korean Journal of Materials Research
  • Journal Title(Ko) :한국재료학회지
  • Volume : 36
  • No :9
  • Pages :325-339
  • Received Date : 2026-06-24
  • Revised Date : 2026-08-20
  • Accepted Date : 2026-09-10
  • DOI :https://doi.org/10.3740/MRSK.2026.36.9.325
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