Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

A.N. Kapitonov; G.N. Alexandrov; F.D. Vasileva; S.A. Smagulova; V.B. Timofeev; N.R. Maksimova; A.A. Kuznetsov

doi:10.3740/MRSK.2016.26.1.1

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Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

January 2016. pp. 1-7

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Abstract

The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at 75°C of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNAdiagnostics.

Keywords

graphene oxide

DNA diagnostics

fluorescent quencher

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Information

Publisher :Materials Research Society of Korea
Publisher(Ko) :한국재료학회
Journal Title :Korean Journal of Materials Research
Journal Title(Ko) :한국재료학회지
Volume : 26
No :1
Pages :1-7
Received Date : 2015-09-07
Revised Date : 2015-11-09
Accepted Date : 2015-11-09
DOI :https://doi.org/10.3740/MRSK.2016.26.1.1

Korean Journal of Materials Research ISSN:1225-0562(Print) 2287-7258(Online) 한국재료학회지

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