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2024 Vol.34, Issue 2

Review

Slipchip Device Development in Molecular Diagnostics
Qingtian Yin1
,
Huiwen Bai2
,
Ruijie Li3†
,
Youngung Seok4†
1Department of Mechanical and Aerospace Engineering, NYU Tandon School of Engineering, Brooklyn, NY 11201, USA
2Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, USA
3Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
4Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea
†Corresponding author E-Mail : rjli@mail.ipc.ac.cn (R. Li, TIPC, CAS) stonehero@jnu.ac.kr (Y. Seok, Chonnam Nat’l Univ.)
27 February 2024. pp. 63-71
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Abstract

Slipchip offers advantages such as high-throughout, low cost, and simple operation, and therefore, it is one of the technologies with the greatest potential for high-throughput, single-cell, and single-molecule analyses. Slipchip devices have achieved remarkable advances over the past decades, with its simplified molecular diagnostics gaining particular attention, especially during the COVID-19 pandemic and in various infectious diseases scenarios. Medical testing based on nucleic acid amplification in the Slipchip has become a promising alternative simple and rapid diagnostic tool in field situations. Herein, we present a comprehensive review of Slipchip device advances in molecular diagnostics, highlighting its use in digital recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and polymerase chain reaction (PCR). Slipchip technology allows users to conduct reliable droplet transfers with high-throughput potential for single-cell and molecule analyses. This review explores the device’s versatility in miniaturized and rapid molecular diagnostics. A complete Slipchip device can be operated without special equipment or skilled handling, and provides high-throughput results in minimum settings. This review focuses on recent developments and Slipchip device challenges that need to be addressed for further advancements in microfluidics technology.

Keywords
Slipchip
diagnostics
recombinase polymerase amplification
loop-mediated isothermal amplification
polymerase chain reaction
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Information
  • Publisher :Materials Research Society of Korea
  • Publisher(Ko) :한국재료학회
  • Journal Title :Korean Journal of Materials Research
  • Journal Title(Ko) :한국재료학회지
  • Volume : 34
  • No :2
  • Pages :63-71
  • Received Date : 2024-01-23
  • Revised Date : 2024-01-23
  • Accepted Date : 2024-01-24
  • DOI :https://doi.org/10.3740/MRSK.2024.34.2.63
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